Saygun, Serkan Ordu Üniversitesi - Fatsa Deniz Bilimleri Fakültesi - Balıkçılık Teknolojisi Mühendisliği Bölümü, Turkey , Bircan, Recep Sinop Üniversitesi - Su Ürünleri Fakültesi - Su Ürünleri Yetiştiriciliği Bölümü, Turkey

The first caryological description of European Flounder, Platichthys flesus luscus (Pallas, 1814) (Pisces, Pleuronectiformes) living in the Black Sea

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In this study, the chromosome structures, numbers and other cytogenetic features of flounder, Platichthys flesus luscus (Pallas, 1814), which is one of the flatfish species in the Black Sea, were examined with using different banding methods. Fish samples were obtained from fishermen in 5 localities between Zonguldak and Ordu of the West and Middle Black Sea coasts. A total of 0.05 % of colchicine solution (25-50 μg / g body) weight was injected from intramuscular to dorsal and from intraperitonal to ventral for big fish (body weight 250 to 1500 g). However, small fish (body weight 50 to 250 g) were maintained in 0.005 % of colchicine solution for 6 hours. Gill and fin epithelia tissues derived by dissecting were incubated in 0.4 % of KCl solution for 30 mins and treated with Carnoy’s fixative. Dried slides were stained with giemsa (pH 6.8) solution for 15 mins.It was determined that the chromosome number of P. f. luscus were 2n=48 and all chromosomes were acrocentrics(number of fundamental was NF=48).In all examined species, there were no anomalies and evidences of sex chromosomes by G-bandings.

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Platichthys flesus luscus , chromosome , karyotype , giemsa staining

Ege Journal Of Fisheries an‎d Aquatic Sciences

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