Author/Authors :
Hür, E Ege Üniversitesi - Tıp Fakültesi - Nefroloji Bilim Dalı, Turkey , Ertilav, M Ege Üniversitesi - Tıp Fakültesi - Nefroloji Bilim Dalı, Turkey , Bozkurt, D Ege Üniversitesi - Tıp Fakültesi - Nefroloji Bilim Dalı, Turkey , Arda, B Ege Üniversitesi - Tıp Fakültesi - Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Anabilim Dalı, Turkey , Sözmen, E Y Ege Üniversitesi - Tıp Fakültesi - Biyokimya Anabilim Dalı, Turkey , Sen, S Ege Üniversitesi - Tıp Fakültesi - Patoloji Anabilim Dalı, Turkey , Basçı, A Ege Üniversitesi - Tıp Fakültesi - Nefroloji Bilim Dalı, Turkey , Akçiçek, F Ege Üniversitesi - Tıp Fakültesi - İç Hastalıkları Anabilim Dalı, Turkey , Duman, S Ege Üniversitesi - Tıp Fakültesi - İç Hastalıkları Anabilim Dalı, Turkey
Abstract :
Aim: Ultrafiltration (UF) failure is usually associated with peritoneal dialysis (PD) peritonitis. Octreotide (OCT) has antiproliferative effects on many cells. The present study aimed to investigate the effect of OCT on antibiotic treatment of peritonitis.Material and Methods: Forty non-uremic female rats, were divided into four groups receiving no treatment (Control, n=12); a peritonitis group which received 1.5ml suspension of E.Coli (10^7CFU/ml)(E.Coli, n=12); an antibiotic group which received 1.5ml suspension of E.Coli (10^7CFU/ml)+antibiotic (AB, cefazoline 50 mg/kg)(E.Coli +AB, n=8) and a OCT group which received E.Coli+AB+50μg/kg OCT intraperitoneally (E.Coli+AB+OCT, n=8). After six hours, a one-hour PET was performed with 3.86% glucose PD solution; and D1/D0 glucose, UF volume, dialysate cell count, dialysate protein and cytokines were determined. A bacteriological culture was performed for all dialysates. Results: E.Coli exposure causes peritonitis with an increase in permeability leading to UF failure. Antibiotic treatment partially preserves UF capacity. Peritonitis induced UF failure which was completely prevented by AB plus OCT treatment. Both treatments led to attenuation of UF failure by inhibiting local TGF b-1 production and decreasing dialysate cell count.Conclusion: In conclusion, adding OCT in dialysate, may help to prevent peritonitis induced UF failure and preserve peritoneal viability by inhibiting acute cytokines over-expression and cell infiltration during peritonitis. In the long-term, it can decrease peritoneal fibrosis.
NaturalLanguageKeyword :
Octreotide , peritonitis , rat model , ultrafiltration failure
