Author/Authors :
Yılmaz Dilsiz, Ö Ege Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, Turkey , Akay, H S T.C. Sağlık Bakanlığı-İzmir Katip Çelebi Üniversitesi - Atatürk Eğitim ve Araştırma Hastanesi - İç Hastalıkları Gastroenteroloji Servisi, Turkey , Ateş, U Ege Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, Turkey , Özütemiz, Ö Ege Üniversitesi - Tıp Fakültesi - İç Hastalıkları Anabilim Dalı, Gastroenteroloji Bilim Dalı, Turkey , Baka, M Ege Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, Turkey
Title Of Article :
Histological evaluation of experimental toxic cirrhosis and omentectomised toxic cirrhosis over rat ovary, fallopian tubes and uteri
Abstract :
Aim: The in vitro model of dimethylnitrosamine toxicity-induced early liver fibrogenesis provides a physiologic milieu to study mechanisms underlying infertility.Materials and Methods: Effects on fertility after injections of dimethylnitrosamine were assessed by light microscopy and fibronectin immunohistochemistry. Rats were randomly allocated to: Group 1, omentectomised and dimethylnitrosamine-induced toxic cirrhosis; Group 2, non-omentectomised and dimethylnitrosmine-induced toxic cirrhosis; Group 3,omentectomised and saline injected sham controls ; and Group 4, controls.Results: Fibrotic alterations of the cirrhotic liver disease were demonstrated with histology. The ovaries for groups 1 and 2 showed cycle arrest and oocyte quality revealed ovoid morphology. Groups 3 and 4 showed unapparent changes. Inspection for fibronectin in the ovarian stroma and follicles revealed decreased immunoexpression for groups 1 and 2. Tuba uterina displayed epithelial thickenning for groups 1 and 2. Normal fallopian and uterine histology with no significant alterations for omentectomy were described for groups 3 and 4.Conclusion: For complete understanding the mechanisms by which environmental chemicals affect intrafollicular processes, further investigation is needed. When no other medication is available for increasing ascites, omentectomy is offered as a valuable surgical tool. Yet this Dimethylnitrosamine-induced cirrhotic model and its relevant findings are keys for following experimental research studies in reversing the effects and for developing drugs or new theraupetic approaches.
NaturalLanguageKeyword :
Omentum , dimethylnitrosamine , liver cirrhosis , infertility , rat , histology
JournalTitle :
Ege Journal Of Medicine
