Author/Authors :
Avcı, Ç B Ege Üniversitesi Tıp Fakültesi - Tıp Fakültesi - Tıbbi Biyoloji Anabilim Dalı, Turkey , Süslüer Yılmaz, S Ege Üniversitesi - Tıp Fakültesi - Tıbbi Biyoloji Anabilim Dalı, Turkey , Şığva Doğan, Ö Ege Üniversitesi - Tıp Fakültesi - Tıbbi Biyoloji Anabilim Dalı, Turkey , Söğütlü, F Ege Üniversitesi - Fen Fakültesi - Biyoloji Bölümü, Turkey , Dündar, M Ege Üniversitesi - Fen Fakültesi - Biyoloji Bölümü, Turkey , Gündüz, C Ege Üniversitesi - Tıp Fakültesi - Tıbbi Biyoloji Anabilim Dalı, Turkey
Abstract :
Aim: Rapamycin is an antibiotic isolated from Streptomyces hygoroscopicus and binds with FKBP12 mTOR. Rapamycin inhibits proliferation of cancer cells in both in vitro and in vivo. It was purposed that cytotoxic, apoptotic effects be taken into consideration and the impact of telomerase activity, EGFR and VDR gene expressions were evaluated.Materials and Methods: LNCaP, PC-3 and DU 145 prostate cancer cell lines were treated with rapamycin in Dulbecco’s Modified Eagle’s Medium. Cytotoxicity of the cell lines were analyzed with the Trypan Blue Dye test, XTT method and apoptosis with acridine orange-ethidium bromide staining. Quantification of hTERT, EGFR and VDR gene expressions was performed.Results: Apoptosis was increased in cell lines with the IC50 dose of rapamycin. When the cyototoxicity of rapamycin was evaluated in the LNCaP cell line, a prominent cyototoxicty was observed. hTERT mRNA expressions decreased in the PC3 cell line. When the gene expressions of VDR and EGFR were analyzed, a distinctive reduction was observed in the VDR gene expression in the PC-3 and DU 145 cell lines. In terms of the EGFR gene expression; a decrease was determined in all cell lines. The most remarkable result of our scientific research is the lack of the VDR gene expression in the LNCaP cell line.Conclusion: The prospect of a new molecular approach to treatment of prostate cancer was raised.
NaturalLanguageKeyword :
Rapamycin , prostate cancer , hTERT , EGFR , VDR
