ÇİÇEK, Candan Ege Üniversitesi - Tıp Fakültesi - Tıbbi Mikrobiyoloji Anabilim Dalı, Turkey , ARSLAN, Ayşe 1Ege Üniversitesi - Tıp Fakültesi - Tıbbi Mikrobiyoloji Anabilim Dalı, Turkey , SAZ, Eylem Ulaş Ege Üniversitesi - Tıp Fakültesi - Çocuk Sağlığı ve Hastalıkları Anabilim Dalı, Turkey , PULLUKÇU, Hüsnü Ege Üniversitesi - Tıp Fakültesi - Enfeksiyon Hastalıkları Anabilim Dalı, Turkey , ÇOK, Gürsel Ege Üniversitesi - Tıp Fakültesi - Göğüs Hastalıkları Anabilim Dalı, Turkey

Simultaneous detection of respiratory viruses using multiplex PCR method in patients with upper and lower respiratory tract infection

14891

Aim: In this study, we aimed to determine the respiratory viruses in a short time by multiplex PCR method in patients with upper and lower respiratory tract infections. Materials and Methods: In this report, between January 2007 and August 2013, a total of 1708 patients (1220 children, 488 adult; 45,1% female, 54.9% male, age range: 5 days-94 years) prediagnosed as having upper or lower respiratory tract infections were enrolled in the study. Respiratory viruses were investigated by multiplex PCR method in respiratory samples of these patients.Results: In a total of 1708 patients, 620 (36.3%) of them were positive for one or more respiratory viruses and 1088 (63.7%) were negative. In 63 of the patients (3.7%) multiple viruses were detected. Most of the samples (89.5%) containing multiple agents were detected in children. Respiratory specimens were obtained from 387 (22.7%) outpatients and 1321 (77.3%) inpatients. One or more respiratory viruses were detected in 39.2% of pediatric patients, 29.1% of adult patients (p 0.000), 48.5% of outpatients and 32.7% of inpatients. In all samples, influenza A(H1N1)pdm09 virus, RSV, rhinovirus and influenza A virus were found be positive in 12.1%, 9.0%, 3.7% and 3.0%, respectively. Conclusion: Respiratory viruses were positive 40% in patients with acute respiratory tract infections. Multiple viral infections were detected in approximately 4% patients. Almost 90% of these patients were found to be of the pediatric patients. If influenza A(H1N1)pdm09 virus is kept separate, the three most common pathogen were RSV, rhinovirus and influenza A virus, respectively. Respiratory viruses were detected more frequently in children compared to adult patients and in outpatient compared to hospitalized patients.

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Multiplex PCR , respiratory viruses

Ege Journal Of Medicine

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