Author/Authors :
OLTULU, Fatih Merkezefendi Devlet Hastanesi - Histoloji ve Embriyoloji Kliniği, Turkey , AKTUĞ, Hüseyin Ege Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, Turkey , UYSAL, Ayşegül Ege Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, Turkey , ÖKTEM, Gülperi Ege Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, Turkey , TURGAN, Nevbahar Ege Üniversitesi - Tıp Fakültesi - Tıbbi Biyokimya Anabilim Dalı, Turkey , KARABAY YAVAŞOĞLU4, Nefise Ülkü Ege Üniversitesi - Fen Fakültesi - Biyoloji Bölümü, Turkey , YAVAŞOĞLU, Altuğ Ege Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, Turkey
Abstract :
Aim: In this study, it was aimed to investigate the possible histological changes in the ovarian structure and particularly in the oocytes, cumulus cells and granulosa cells containing thyroid hormone receptors, tissue regeneration capability and capacity in terms of stem cell markers immunohistochemically by experimentally-induced thyrotoxicosis in rats. Materials and Methods: Two study groups were formed (eight rats for each group); 500μgr/kg/day 3,3 ,5-Triiodo-L- thyronine was injected intraperitoneally to female rats in thyrotoxicosis group during ten days. In 11th day, all animals were anesthesized, 5 cc blood was drawn for biochemical evaluation and then all were decapitated in order to remove ovaries. Ovaries were placed in 4% paraformaldehyde fixative for immunohistochemical investigation. Immunohystochemical processing and staining were performed routinely and all samples were evaluated by light microscopy.Results: As a result of immunohistochemical staining, c-kit and Thy-1 expressions were found to be significantly decreased in the thyrotoxicosis group compared with the control group, whereas Nanog expression was increased in the thyrotoxicosis group. Decrease in c-kit and Thy-1 expression in the thyrotoxicosis group caused a reduction in the stimulatory effects of the growth factors on oocytes, cumulus and granulosa cells.Conclusion: Decreased levels of c-kit and Thy-1 in rat model of thyrotoxicosis lead to a decrease in stromal growth support and deterioration of microenvironment. Elevated levels of Nanog expression in thyrotoxicosis was interpreted as an effort to protect the microenvironment for the sake of follicles and oocytes despite the increase in catabolic processes (as a result of increased metabolism in almost all cells due to thyroid hormones)
NaturalLanguageKeyword :
c , Kit , stem cell , Nanog , ovary , thyrotoxicosis
