Author/Authors :
Turhan, Ajda Ege Üniversitesi - Tıp Fakültesi, Kan Merkezi, Turkey , Altuğlu, İmre Ege Üniversitesi - Tıp Fakültesi - Tıbbi Mikrobiyoloji Anabilim Dalı, Turkey , Erensoy, Selda Ege Üniversitesi - Tıp Fakültesi - Tıbbi Mikrobiyoloji Anabilim Dalı, Turkey , Sertöz, Rüçhan Ege Üniversitesi - Tıp Fakültesi - Tıbbi Mikrobiyoloji Anabilim Dalı, Turkey
Abstract :
Aim: Hepatitis B virus (HBV) DNA quantification is an important indicator during the follow-up of chronic hepatitis B infection, which has a major public health impact in Turkey due to its hepatocellular carcinoma and fatal liver damage end-points. There are many different methods and systems used in clinical virology laboratories for HBV DNA quantification. The aim of this study was to test whether a laboratory designed real time assay protocole would be compatible with ABI Prism 7500 (PE Biosystems) in Ege University Medical School’s Virology/Molecular Biology laboratory and to compare it with the routinely used COBAS AmpliPrep-COBAS TaqMan 48 (CAP-CTM, Roche, Branchburg, NJ) HBV DNA test. Materials and Methods: A real time test based on Taqman technology to detect HBV DNA was designed in the Virology/Molecular Biology Laboratory of Ege University Medical School’s Medical Microbiology Department and it was evaluated. 332 samples sent to the laboratory for HBV DNA quantification were included in the study and were compared with the COBAS AmpliPrep-COBAS TaqMan 48 HBV DNA test routinely used in the laboratory. Results: HBV DNA results of 176 out of 332 samples were in the dynamic range of the protocol. The quantitative results of 176 samples in dynamic range were concordant. The results of 106 samples were negative according to both systems.Conclusion: The laboratory designed HBV DNA quantification protocole can be used in the clinical virology laboratory for the routine diagnosis and follow-up of HBV infected patients and it is a suitable and cheap method enabling a wide dynamic range
