Author/Authors :
Türkdogan, Kenan Ahmet Isparta Devlet Hastanesi - Acil Departmani, Turkey , Güven, Fatma Mutlu Kukul Cumhuriyet Üniversitesi - Tip Fakültesi - Acil Tip AD, Turkey , Türkdogan, Figen Tunali Isparta Devlet Hastanesi - Radyoloji Bölümü, Turkey , Karabacak, Mustafa Isparta Devlet Hastanesi - Kardiyoloji Klinigi, Turkey , Orhan, Hikmet Süleyman Demirel Üniversitesi - Biyoistatistik ve Tip Bilisimi AD, Turkey , Polat, Zübeyde Akin Cumhuriyet Üniversitesi - Tip Fakültesi - Parazitoloji AD, Turkey , Karahan, Oguz Dicle Üniversitesi - Kalp Damar Cerrahisi AD, Turkey
Abstract :
Objective: This study investigated the effects of a Rho kinase inhibitor on vascular endothelial cells. Material and Methods: Human umbilical venous endothelial cells (HUVECs) were obtained from the American Type Culture Collection. Cells were seeded in 96-well gelatin coated microtiter plates at a concentration of 1×10^4 cells/ml in a final volume of 100 µl per well. Three groups were established: a control group and groups with rho kinase inhibitor (fasudil) added at concentrations of 5 or 6 mM. These baseline cultures were then observed for 72 hours. Results: A strong linear cell proliferation was seen in the control and the rho kinase inhibitor groups. The average concentration was 1.063 for the 5 mMol fasudil group and 1.147 for the 6 mM group, and this difference was statistically significant (p=0.044). The number of cells analyzed at 24 to 48 hours showed a significant difference, but the cell numbers between 48 and 72 hours did not differ statistically. Conclusion: Fasudil did not show any cytotoxicity to endothelial cells; on the contrary, it promoted cell proliferation. The increase in endothelial cell proliferation and vascular stenosis was caused by neointimal hyperplasia, which would be detrimental in diseases such as stent stenosis.
