Ak, Cennet Süleyman Demirel Üniversitesi - Tıp Fakültesi - Fizyoloji A D, Turkey , Bayram, Dilek Süleyman Demirel Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji A D, Turkey , Armağan, İlkay Süleyman Demirel Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji A D, Turkey , Uğuz, A. Cihangir Süleyman Demirel Üniversitesi - Tıp Fakültesi - Biyofizik A D, Turkey

An investigation on the effect of leptin on rat kidney tissue

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Aim: increasing day by day obesity, one of the preventable health problems. Obese individual increases the risk of renal disease one and a half times more compared to the non-obese individuals. Leptin is a type of adipokinin hormone which is released from adipose tissue cells in proportion to the amount of adipose tissue. It is known that leptin leads to a rise in reactive oxygen radicals in the tissue. This study aimed at investigating if leptin affects renal tissue by giving leptin to the rats. Material–Method: The total of 16 Wistar albino rats (weights ranged from 250g-300g) in the study were randomly divided into groups of eight in two different cages. The first group was the Control group (intraperitoneally = i.p. 0.9% normal saline) and the second group was the leptin group (ip 0,1 mg/kg leptin). The subjects, which were given leptin i.p. for 7 days, and the ones in the control group, which were given 0.9% normal saline to trigger the same stress, were sacrificed and their kidneys were taken out for examination. The kidneys were fixed with 10% neutral form was formaldehide to routine histological procedure. The sections stained with hematoxylin-eosin = H-E were analyzed under light microscopy. Results: The sections in the control group were found to be normal hystologically. Compared to these, the renal tissue samples in the leptin group were determined to have mononuclear cell infiltration in the cortex, tubular dilatation in the medulla, degeneration and hemorrhagic areas, vacuolar degeneration in glomeruli and dilation in proximal and distal tubular. Discussion: Revealing the effects of leptin on renal damage is important for avoiding harmful effects of obesity through hormonal and behavioral arrangements. This study suggested that leptin could cause renal pathologies by damaging renal tissue. We are of the opinion that being able to explain renal damage etiology exactly will be useful to differential diagnosis and treatments.

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Leptin , kidney , obesity , renal damage , adipokines

Medical Journal Of Suleyman Demirel University

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