Author/Authors :
OLGUN, Murat Eskisehir Osmangazi University - Faculty of Agriculture, Turkey , AYTER, N. Gözde Eskisehir Osmangazi University - Faculty of Agriculture, Turkey , BUDAK BAŞÇİFTÇİ, Zekiye Eskisehir Osmangazi University - Faculty of Agriculture, Turkey , TURAN, Metin Yeditepe University - Faculty of Engineering and Architecture - Department of Genetics and Bioengineering, Turkey , KOYUNCU, Onur Eskisehir Osmangazi University - Faculty of Science, Turkey , ARDIÇ, Murat Eskisehir Osmangazi University - Faculty of Science, Turkey , AĞAR, Güleray Atatürk University - Faculty of Science, Turkey , TAKIL, Engin Eskisehir Osmangazi University - Faculty of Agriculture, Turkey
Abstract :
The objective of this study was to determine genetic distance between some wheat varieties based on RAPD and ISSR analyses. The number of amplified bands of genotypes in the primers ranged 3-10 in RAPD and 6-12 in ISSR. The most polymorphic bands in primer/primer combinations were obtained from OPBA-03, OPL-15 and OPY-13 in RAPD, from UBC810 in ISSR. Varieties were classified about in three groups based on the dendogram results. Yunus and Altay-2000, Nacibey and Sultan-95, Harmankaya and Soyer were found to be close related varieties, whereas distant-related varieties were Alpu-01 and Nacibey. RAPD and ISSR are efficiently used to evaluate genetic variation, to determine genetic diversities and improvement and development of novel varieties.
NaturalLanguageKeyword :
Wheat , genetic divergence , DNA extraction , genetic polymorphisms , RAPD and ISSR analyses
