Author/Authors :
OLGUN, Murat Eskisehir Osmangazi University - Faculty of Agriculture, Turkey , BUDAK BASÇIFTÇI, Zekiye Eskisehir Osmangazi University - Faculty of Agriculture, Turkey , AYTER, N. Gözde Eskisehir Osmangazi University - Faculty of Agriculture, Turkey , TURAN, Metin Yeditepe University - Faculty of Engineering and Architecture - Department of Genetics and Bioengineering, Turkey , KOYUNCU, Onur Eskisehir Osmangazi University - Faculty of Science, Turkey , ARDIÇ, Murat Eskisehir Osmangazi University - Faculty of Science, Turkey , AGAR, Güleray Atatürk University - Faculty of Science, Turkey , TAKIL, Engin Eskisehir Osmangazi University - Faculty of Agriculture, Turkey
Abstract :
Abstract: The objective of this study was to determine genetic distance between barley genotypes based on RAPD and ISSR analyses. The number of amplified bands of genotypes in the primers ranged 4-11 in RAPD and 7-11 in ISSR. The most polymorphic bands in primer/primer combinations were obtained from OPA-04, OPA-13 and OPH-17 in RAPD, and UBC-808, UBC-820 and UBC-872 in ISSR. Results showed that average polymorphic rate was 86.5%. Performances of cultivars in groups asist possibility in creating genetic variability in barley cultivar development. RAPD and ISSR methods are useful for evaluation of genetic diversity and could be safely used to determine the genetic relationships in barley genotypes.
NaturalLanguageKeyword :
Barley , genetic divergence , DNA extraction , genetic polymorphisms , RAPD and ISSR
