In vitro Adventitious Shoot Regeneration from Leaves and Petiols of Gerbera (Gerbera jamesonii Bolus)

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In this study, effects of plant growth regulators on shoot regeneration from leaf and petiol explants of Gerbera cultivar ‘Rosalin’ were determined. For this purpose, 18 different combinations of plant growth regulators consisting of three doses of AgNO_3 (0, 1.0 and 2.0 mg/L) and three doses of BAP (1.0, 2.0 and 3.0 mg/L) or thidiazuron (0.3, 0.6 and 1.2 mg/L) were tested. In addition, 0.1 mg/l NAA was added to all MS media. In the study, TDZ applications gave better results based on the formation of callus for both explants types. When the leaves were used as explants, no shoot formation was observed on MS medium containing both BAP and TDZ. When the petiole was used as explants, BAP applications gave better results than TDZ application in term of shoot regeneration. In the study, the highest shoot regeneration rate, shoot number and leaf number were 22.5%, 2.0 shoots, and 3.33 leaves on the MS medium containing 3 mg/l BAP + 0.1 mg/l NAA, respectively. Applications of silver nitrate were not effective on in vitro shoot regeneration in gerbera.

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Gerbera , benzylaminopurine , silver nitrate , thidiazuron , shoot regeneration.

Journal Of The Faculty Of Agriculture, Suleyman Demirel University

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Journal Of The Faculty Of Agriculture, Suleyman Demirel University