Author/Authors :
ŞİMŞEK, Fikri Selçuk Elazığ Eğitim ve Araştırma Hastanesi - Nükleer Tıp, Türkiye , KOCAMANa, Nevin Fırat Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji, Türkiye
Abstract :
Objective: Current medical practice commonly use radiological imaging techniques for the diagnosis, staging, evaluating and response to treatment. However most of these imaging modalities utilize radiation, which is known to result in increased oxidative stress. In this study, we aimed to evaluate the impact of imaging modalities involving radiation to the immune reactivity of ghrelin in the liver. Material and Method: Total 20 female Wistar albino rats were divided into 4 equal groups. Animals in group I comprised the controls. Rats in Group II underwent a CT examination at 0 hour with a dose 50 mA and 110 keV, Group III received single dose, 1 mci FDG at 0 hour, followed by a PET/CT examination after 1 hour. Rats in Group IV were administered i.v. FDG 1 mci at 0 hour. All animals were decapitated at 24. hours. Subsequently, liver tissue samples were appropriately immune-stained, examined under light microscopy, statistical analysis were performed and photographs obtained during microscopical examination. Results: In comparison with liver tissue from control rats, no significant differences were found in the ghrelin immunereactivity in rats receiving FDG only. On the other hand, statistically significant increases were observed in CT and PET/CT imaging groups. However, between CT and PET/CT groups didn’t differ significantly. Conclusion: Our results suggest that CT and PET/CT imaging are associated with a significant increase in rat liver ghrelin immunereactivity, while FDG alone has no such effect.
