Author/Authors :
ŞENER, Azize Marmara Üniversitesi - Eczacılık Fakültesi - Biyokimya Anabilim Dalı, TÜRKİYE , GÜMÜŞ, Ayşegül Marmara Üniversitesi - Eczacılık Fakültesi - Biyokimya Anabilim Dalı, TÜRKİYE , GÖKER, Bahar Marmara Üniversitesi - Eczacilik Fakültesi - Biyokimya Anabilim Dali, TÜRKİYE , ARBAK, Serap Acıbadem Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, TÜRKİYE , ÖZSAVCI, Derya Marmara Üniversitesi - Eczacılık Fakültesi - Biyokimya Anabilim Dalı, TÜRKİYE , YURTSEVER, Ertuğrul Marmara Üniversitesi - Eczacılık Fakültesi - Biyokimya Anabilim Dalı, TÜRKİYE
Abstract :
Objective: In this study, it has been investigated whether there are antioxidative and hepatoprotective effects of ethanol extract of Urtica dioica L. seeds on CCl4 induced hepatotoxicity in rats.Materials and Methods: In our study, Wistar albino type rats were used. Four groups have been formed as control group, CCl4 group, extract group and extract+CCl4 group. The extract prepared by ethanol extraction of Urtica dioica was administered i.p. for 14 days. Out of biochemical parameters, ALT and AST levels were measured. Liver and plasma lipid peroxide (LPO) and glutathione (GSH) levels were measured and also histological studies were performed.Results: A significant increase has been detected in plasma ALT, AST and LPO levels of CCl4 group compared to the control group. LPO, ALT and AST levels of extract+CCl4 group significantly decreased. The extract administration decreased the elevation in CCl4 induced liver LPO levels compared to the control and CCl4 groups whereas LPO levels remained at control group levels.Only the liver GSH levels of extract administered rats increased by %21 compared to the control group. GSH levels of extract+CCl4 group did not change compared to the CCl4 group. The results of the biochemical tests were confirmed with the histopathological analysis. The liver structure has been observed close to normal only in the extract administered group but CCl4 administration caused severe damage in the hepatocytes. It has been observed that CCl4 induced liver damage has decreased in the extract+CCl4 group.Conclusion: The extract has been shown to have hepatoprotective effect by decreasing the hepatotoxic effect of CCl4.
NaturalLanguageKeyword :
Urtica dioica L. , antioxidant , hepatoprotective effect , lipid peroxidation
