çakir, murat yozgat bozok üniversitesi - tıp fakültesi - fizyoloji anabilim dalı, Yozgat, Turkey , tekin, suat inönü üniversitesi - tıp fakültesi - fizyoloji anabilim dalı, Malatya, Turkey , beytur, asiye inönü üniversitesi - tıp fakültesi - fizyoloji anabilim dalı, Malatya, Turkey , sandal, süleyman inönü üniversitesi - tıp fakültesi - fizyoloji anabilim dalı, Malatya, Turkey

Investigation of the Effects of N- (p-amylcinnamoyl) Anthranilic Acid (ACA) on Human Over and Prostate Cancer Cell Viability

17955

Objective: Prostate and ovarian cancers are among the most common types of cancer in Turkey. N- (p-amylcinnamoyl) anthranilic acid (ACA) is an inhibitor of both transient receptor potential melastatin-2 (TRPM2) channels and phospholipase A2 (PLA2). Recent studies have reported that TRPM2 channels and PLA2 are associated with cancer. The aim of the study is to investigate anti- cancer mechanism of ACA in ovarian (A2780) and prostate (PC-3 and LNCaP) cancer cells. Materials and Methods: A2780, PC-3 and LNCaP cell lines were used in the study. All cell lines were incubated with ACA at concentrations of 1 μM, 5 μM, 25 μM, 50 μM and 100 μM for 24 h. Changes in cell viability were examined by 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) method. The inhibitory logarithmic concentration 50 (LogIC50) values were calculated according to MTT assay results. Results: There was a significant decrease in survival of cancer cells (A2780, PC-3 and LNCaP) incubated with ACA for 24 hours (P 0.05). All concentrations of ACA added to the culture medium caused a decrease in A2780 cell viability (P 0.05), In PC-3 and LNCaP cells, all concentrations of ACA except 1 μM were found to reduce cell viability for 24 hours (P 0.05). Furthermore, when LogIC50 values were examined, it was determined that ACA showed the strongest cytotoxic activity on LNCaP cells at lower concentration. Conclusion: ACA was found to have cytotoxic activity on the A2780, PC-3 and LNCaP cells. These findings suggest that ACA has antitumor properties.

87

N , (p , amylcinnamoyl) antranilik asit (ACA) , transient receptor potential melastatin , 2 (TRPM2) channels , phospholipase A2 , cancer

Fırat University Medical Journal Of Health Sciences

91