Determination of Nucleotide Partial Sequence of VP2 Region of Infectious Pancreatic Necrosis Virus Isolated from Ovarian Fluid of Rainbow Trout (Oncorhynchus mykiss, Walbaum 1792) in Turkey

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In this study, partial nucleotide sequence VP2 region of Infectious Pancreatic Necrosis Virus (IPNV) viral protein was detected from isolated ovarian fluid of rainbow trout (Oncorhynchus mykiss Walbaum,1792) and its genetic relation was investigated to the worldwide IPNV isolates. For this purpose, IPNV was isolated from ovarial fluid from fish cages belon to private enterprises in Elaziğ province and identified by RT-PCR method and subjected to partial nucleotide sequence for VP2 region viral protein for this isolate. The nucleotide and deduced amino acid sequence data reported in this isolate have been deposited in GenBank with the following accession number: KC525214 ELAZIĞ. Phylogenetic analysis was made for this isolate. Phylogenetic analysis revealed that KC525214 ELAZIĞ isolate was clustering within Genogroup 5 clade most closely related to the Serotype A2 together with French, Norwegian, Danish, Taiwanese, Spanish and American isolates. The findings indicated that the transfer of ovarian fluids is associated with the transmission and spread of IPNV. The determination of partial nucleotide sequence VP2 region viral protein for IPNV was performed with this study in Turkey. These results are of importance for further epidemiological studies and IPNV outbreaks in Turkey.

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Infectious pancreatic necrosis virus , ovarian fluid , Rainbow trout , nucleotide sequence , viral protein VP2 region

Fırat University Veterinary Journal Of Health Sciences

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Fırat University Veterinary Journal Of Health Sciences