Author/Authors :
GÜLTİKEN, Nilgün Ondokuz Mayıs Üniversitesi - Veteriner Fakültesi - Doğum ve Jinekoloji Anabilim Dalı, Turkey , YARIM, Gül Fatma Ondokuz Mayıs Üniversitesi - Veteriner Fakültesi - Biyokimya Anabilim Dalı, Turkey , YARIM, Murat Ondokuz Mayıs Üniversitesi - Veteriner Fakültesi - Patoloji Anabilim Dalı, Turkey
Title Of Article :
Determination of Serum Adenosine Deaminase Activity in Bitch Diagnosed as Cystic Endometrial Hyperplasia (CEH)-pyometra Complex
Abstract :
The purpose of the study was to investigate serum ADA activity and blood lymphocyte and monocyte numbers in the bitch diagnosed as cystic endometrial hyperplasia (CEH)-pyometra complex. Twenty five female dogs were involved in the study. Samples from 18 bitches with open cervix CEH-pyometra and 7 mid-diestrus bitches were used. The diagnosis of CEH-pyometra was based on clinical, ultrasonographical and haematological findings. Mid-diestrus control bitches were diagnosed by vaginal cytologic examination. Blood samples were taken before the operation and tissue samples were collected at the time of surgery from the uteri of 18 adult bitch diagnosed as cystic endometrial hyperplasia (CEH)-pyometra complex and of 7 healthy mid-diestrus dogs presented for ovariohysterectomy for histopathological investigation. Serum ADA activity was measured by spectrophotometrically and determined to be 5.99±0.31 U/l in bitches with CEH- pyometra and 3.50±0.64 U/l in mid-diestrus bitches. Mean serum ADA activity were higher in bitches with CEH-pyometra complex compared with the concentrations in healthy mid-diestrus dogs (p≤0.01). It was concluded that CEH-pyometra complex results in increase in serum ADA activity by cell-mediated immune system and evaluation of serum ADA activity could be an useful parameter for diagnosis and treatment follow-up of this complex.
NaturalLanguageKeyword :
ADA , Cystic Endometrial Hyperplasia , Bitch , Pyometra Complex
JournalTitle :
Kocatepe Veterinary Journal
