durmuş, ibrahim afyon kocatepe university - suhut vocational school - department of veterinary health and laboratuary, TURKEY , evcimen, mustafa afyon kocatepe university - faculty of veterinaey medicine - department of physiology, TURKEY , salim, mehmet naci afyon kocatepe university - faculty of veterinaey medicine - farm of reseach, TURKEY , küçükkurt, ismail afyon kocatepe university - faculty of veterinary medicine - department of biochemistry, Turkey , ince, sinan university of afyon kocatepe - faculty of veterinary medicine - department of pharmacology and toxicology, Turkey , eryavuz, abdullah afyon kocatepe university - faculty of veterinaey medicine - department of pharmacology and toxicology, Turkey

Determination of Changes in Some Biochemical Parameters and Oxidant-Antioxidant Balance After Food Intake in Sheep

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Daily variations in the concentration of urea, glutamine, glucose, cortisone, malondialdehyde (MDA) and antioxidant potential (AOP) were evaluated in the blood of sheep fed once a day. The study was carried out on 10 Anatolian Merinos sheep, average 1.5 years old and healthy. Animals were fed once per day with 61.3 % forage and 38.7 % concentrate mixture throughout the experimental period for 15 days. The blood samples were taken from the animals at first hour before and at 1, 3, 5, 7, 9, 12, 18 and 24th hours after feeding. Urea concentration decreased at first hour after feeding compared to before feeding and started augmentation after that and reached to levels before feeding at 12th hour after feeding. Glucose concentration increased at first hour after feeding compared to before feeding and started decline after that and reached to levels before feeding at 5th hour after feeding. Glutamine and cortisone concentrations decreased from at first hour to at 7th hour after feeding compared to before feeding and reached to levels before feeding at 7th hour after feeding. MDA levels decreased at first and 5th hours after feeding compared to before feeding and started increasing after 7th hour and reached significantly higher levels than before feeding in samples taken from at 12th hour to at 24th hour after feeding. While AOP increased at 3th and 9th hours after feeding, it decreased at 5, 7 and 12th hours after feeding. We concluded that the blood metabolic profiles and oxidant and antioxidant balance in sheep vary depending on time after feeding. In order to maximize the diagnostic value of these the blood metabolic profiles, the most suitable time for blood collection seems to be just before the feeding in sheep fed once a day.

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Antioxidant , Cortisone , Glutamine , Metabolic profile , Oxidant , Sheep

Kocatepe Veterinary Journal

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