مرکز منطقه ای اطلاع رساني علوم و فناوري - Characterization of Vibrio anguillarum Isolates Recovered From Diseased Cultured European Sea Bass (D. Labrax)

Abstract :

The aim of this study is the bacteriological, molecular and serological characterization and determination of the biofilm formation of Vibrio anguillarum isolates that induced vibriosis in cultured sea bass (D. labrax). In this study, sampling studies were made on 5 fish samples weighing between 150-200 g, showing some disease signs supplied from fish farm. As a result of the bacteriological inoculations on the different media made from the internal organs of the diseased fish samples such as liver, kidney and spleen. Bacterial isolates were identified as V. anguillarum according to their biochemical properties. Isolates that were identified as V. anguillarum formed a single band of 519 bp in the rpoN PCR analysis. In the slide agglutination and Dot-blot tests that were performed by using antibodies raised against V. anguillarum serotypes O1, O2 and O3, O-antigen extracts obtained from V. anguillarum recovered from diseased cultured sea bass in this study, only reacted with the antibody raised against serotype O1. As a result, with this study, serotyping of V. anguillarum by Dot-blot method was examined for the first time in Turkey with this study.