Effect of Carnosic Acid On The Short Term Storage Anatolian Buffalo Epididymal Sperm

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This study was conducted to determine the effect of carnosic acid, extracted from rosemary (rosmarinus officinalis), to epididymal buffalo sperm to short-term motility, abnormal spermatozoon rates and HOS test ratio. Semen samples were obtained from 36 (4-5 year old) Anatolian buffalos after the slaughtering. Semen were collected from each 6 testes they were divided into 5 equal parts and groups were formed and the study was repeated 6 times. The groups were composed with the Tris extender containing carnosic acid 12.5, 25, 50 and 100 μg / ml and no-additive (control). Semen were cooled to +4 °C and spermatological data was evaluated at 0, 24 and 48. hours. Although there was no difference between the groups at the 0.h when evaluated in terms of motility, in 24. h motility values were higher in all groups than control. Besides, 48.h motility values were higher in groups of 12.5, 25 and 50 μg / ml than control. these values were statistically significant (p 0.05). In terms of total abnormal spermatozoon the protective effect of carnosic acidwas found significantlyin 12.5 μg / ml group at 0 h, 12.5 and 25 μg / ml group at 24 h and all groups at 48 h.respectively. With regard H+/E-, protective effect of carnosic acidwas determined in 25 μg/ml group and in all groups at 24 and 48 h. In conclusion, it was determined that the doses of 12.5 and 25 μg / ml of carnosic acid added to extenderin +4 °C storage of buffalo epididymal sperm showed a protective effect compared to the control group in terms of motility, spermatozoon morphology, membrane integrity and viability.

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Epididymal Sperm , Carnosic Acid , Short Term Storage , Buffalo

Kocatepe Veterinary Journal

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Kocatepe Veterinary Journal