Ak, Gülser Gazi Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, Türkiye , Ilgaz, Celal Gazi Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, Türkiye , Erdoğan, Deniz Gazi Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, Türkiye , Helvacıoğlu, Fatma Gazi Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, Türkiye , Bağırzade, Mahmut Gazi Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, Türkiye , Kaplanoğlu, Gülnur Take Gazi Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, Türkiye

Ultrastructural Investigation of Parietal Cells in Fasting-After Fasting and the Effects of Histamin and Gastrin

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Objective: The aim of this study is to determine the ultrastructural effect of fasting and applications of histamine and gastrin after fasting in gastric parietal cell. Methods: In this study, nine groups were composed of Swiss albino male rats. Group 1: Control, group 2: fasting for 12 hours, group 3: 12 hours fasting +1hour histamine, group 4: 12 hours fasting + 3min. gastrin, group 5: 12 hours fasting + 3min. DMSO (solvent of gastrin), group 6: 12 hours fasting + 7min. gastrin, group 7: 12 hours fasting + 7min. DMSO, group 8: 12 hours fasting + 15min. gastrin, group 9: 12 hours fasting + 15min. DMSO. At the end of the study time, the tissue samples were passed through routine electron microscopic preparation methods and tissues were embedded in araldite CY212 kit. Thin sections were evaluated on Carl Zeiss EM 900 electron microscope.Results: It was determined in the executed research, by using the electron microscope, that tubulovesicular structures were increased in the fasting group independently of the control group, vacuolar structures were also observed in patches and histamine application did not reveal any difference except for mitochondrial differences in small amounts. However, it was determined that gastrin application increased the cellular degeneration parallel to increasing duration. This transformation was especially observed in nuclear structures, mitochondria and intracytoplasmic canaliculi in the cells. Conclusion: The distinct degeneration which is observed during the gastrin application in this study was evaluated as a result of the augmentation of the acid secretion by gastrin using two ways.

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Parietal cell , fasting , gastrin , histamin , ultrastructure

Gazi Medical Journal

64