Author/Authors :
çildağ, songül adnan menderes üniversitesi - tıp fakültesi - immünoloji-romatoloji bd, Aydın, Türkiye , korkmazgil, berna adnan menderes üniversitesi - tıp fakültesi - tıbbi mikrobiyoloji abd, Aydın, Türkiye , kara, yasemin adnan menderes üniversitesi - tıp fakültesi - immünoloji-romatoloji bd, Aydın, Türkiye , kale, hacer adnan menderes üniversitesi - tıp fakültesi - iç hastalıkları abd, Aydın, Türkiye , akın, nesim adnan menderes üniversitesi - tıp fakültesi - iç hastalıkları abd, Aydın, Türkiye , şentürk, taşkın adnan menderes üniversitesi - tıp fakültesi - immünoloji-romatoloji bd, Aydın, Türkiye
Abstract :
Purpose:Antinuclear antibodies have been used for years for diagnosis and follow-up of systemic rheumatic diseases. Indirect immunofluorescence assay (IIF) is the gold standard to reveal the presence of ANA (ANA). This study intends to investigate by the IF-ANA method the relation of ANA staining patterns, titration and antibodies types with systemic rheumatic diseases. Materials and methods:The study included 215 patients, who were being followed up in our clinic with the diagnosis of systemic rheumatic disease between September 2015 and December 2015, who had an ANA-positive test and underwent subgroup analysis. ANA was tested using IIF method and ANA profile was tested by using a commercial kit with immunoblotting. ANA staining patterns, titration results, and results of antibodies types were compared with types of systemic rheumatic disease. Results:In the study, the most frequent ANA staining pattern was granular (34.4%) and homogeneous (33.5%) while the most frequent result in the subgroup analysis was R0-52 (28.8%), SS-A (24.1%), and ds-DNA (19.06%) positive. The most frequent ANA titration was 1/100, 1/320, 1/1000 and above, respectively. Conclusion:Although specific antibody positivity is higher in SLE and SSc, the existence of specific antibodies in different percentages in all the rheumatic diseases indicates the importance of using ANA with the clinic findings in the diagnosis and follow-up of systemic rheumatic disease.
NaturalLanguageKeyword :
Anti , nuclear antibodies , indirect immunofluorescence assay , systemic rheumatic diseases
