Comparison of RNA extraction methods for the molecular detection of Artichoke latent virus in globe artichoke

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Globe artichoke is very beneficial vegetable for human health because of involve vitamins and minerals within its structure. This vegetable which is intensively cultivated in the Mediterranean basin, are consumed in the form of meals, soups and salads with its own flavor in Mediterranean cuisine. In recent years, its production and consuming are increasing and spreading all over the world. As other cultivated plants, globe artichoke has been affected by many plant pathogens. In this study, the success of some RNA extraction methods that is very important step for detecting Artichoke latent potyvirus which cause yield and quality loss were investigated. RT-PCR is a reliable method and commonly used for detection and identification of viruses. Globe artichoke is a plant with high content of phenolic acids which make difficult isolation of RNA. Thereby the results of RT-PCR are affected. In this study, our aim is investigation of three widely used extraction methods and a commercial isolation kit’s effectiveness on artichoke with high content of phenolic compounds. The results of this study showed substantially parallel to the literature and also the commercial kit and silica capture methods were found effective to obtain high purity RNA with high concentration.

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Artichoke latent potyvirus , Lithiumchloride , RNA , Extraction , Silica , capture

The Journal Of Ege University Faculty Of Agriculture

297

The Journal Of Ege University Faculty Of Agriculture