özkaynak, ercan yüksel tohum tarım san. ve tic. a. ş., Antalya, Türkiye , şimşek, tuğba yüksel tohum tarım san. ve tic. a. ş., Antalya, Türkiye

Determination of Drought Tolerance on Advanced Potato Lines In Vitro Conditions

23336

This study aim was to determine the effect of some advanced potato lines to drought stress conditions in vitro using PEG 6000. This research was P E G 6000 conducted at the Tissue Culture Laboratory in January 2016 to May 2016. The Randomized Split Block Design was carried out PEG concentrations of three levels, namely: MSO: no PEG, PI: 3% PEG, P2= 10% PEG and P3: 15% PEG with three replications. Plant traits such as plant height, number of nodes per plant and plant fresh weight were measured and evaluated after 20 and 40 days of culture period. In general, the higher values were obtained from MSO (MS + 3% sucrose + 7 g/l agar) compare to PI (MS + 3% sucrose + 7 g/l agar + 3% PEG), P2 (MS + 3% sucrose + 7 g/l agar + 10% PEG) and P3 (MS + 3% sucrose + 7 g/l agar + 15% PEG) for all plant characteristics. The research results showed that increasing the concentration of PEG will reduce plant height, number of nodes per plant, plant fresh weight and plant growth and development. As a result of the research, 13-80-44 and 22-99-33 advanced lines were selected as drought tolerant lines in vitro PEG 6000 induced conditions.

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Advanced line , breeding , drought , potato , PEG 6000

The Journal Of Ege University Faculty Of Agriculture

243