Author/Authors
odaci demirkol, dilek ege university - faculty of science - biochemistry department, Izmir, Turkey , ozdemir, caglar ege university - faculty of science - biochemistry department, Izmir, Turkey , pilloton, roberto institute of atmospheric pollution research of the national council of research (cnr), Rome, Italy , timur, suna ege university - faculty of science - biochemistry department, Izmir, Turkey
Title Of Article
Carbon Nanotube Modified Screen Printed Electrodes: Pyranose Oxidase Immobilization Platform for Amperometric Enzyme Sensors
شماره ركورد
25586
Abstract
Here, a novel enzymatic biosensor was developed using multiwalledcarbon nanotube including screen printed electrodes (MWCNT‐SPE). Pyranoseoxidase (PyOx) was immobilized on the electrode surface by way of gelatinmembrane and then cross‐linked using glutaraldehyde. Glucose was detected at ‐0.7 V (vs. Ag/AgCl) by watching consumed oxygen in enzymatic reaction afteraddition substrate. After optimization of pH and enzyme loading, the linearitywas found in the range of 0.1–1.0 mM of glucose. After that, the effect of MCNT onthe current was tested. Also the enzymatic biosensor including glucose oxidaseinstead of pyranose oxidase was prepared and the biosensor response followedfor glucose. Furthermore, this system was tested for glucose analysis in soft drinks.
From Page
286
NaturalLanguageKeyword
Pyranose oxidase , Multiwalled carbon nanotube , Screen printed electrode , Glucose analysis , Gelatin
JournalTitle
Journal Of Natural and Applied Sciences
To Page
291
JournalTitle
Journal Of Natural and Applied Sciences
Link To Document