Author/Authors :
Küçük, Mutlu Deneysel Tip Arastirma Enstitüsü - Deney Hayvanlari Biyolojisi ve Biyomedikal Uygulama Teknikleri Anabilim Dali, Turkey , Çevik, Aydin Deneysel Tip Arastirma Enstitüsü - Deney Hayvanlari Biyolojisi ve Biyomedikal Uygulama Teknikleri Anabilim Dali, Turkey , Emre, Ali Istanbul Üniversitesi - Istanbul Tip Fakültesi - Genel Cerrahi Anabilim Dali, Turkey
Abstract :
Pancreas transplantation (PTx) is the one of the most effective treatment which is able to restore normoglycemia without exposing diabetic patients to the risk of severe hypoglycemia. It has first applied in 1966 and proceeded in a slow but an increasingly successful way compared to other organ transplantations. In recent years, its success rate has been increased. Experimental studies in clinical applications of pancreas transplantations began in late 19 th century and done in dogs at 1957. A successful pancreas transplantation was followed by many pancreas transplantations done in other animal species for many years. Spontaneous diabetic strains or chemically induced (streptozotocin and alloxan) diabetes or total pancreatectomized laboratory animals (especially dogs and rats) have been used for experimental pancreas transplantations. All modifications in rat models of PTx are the result of changes of three important points: the arterial inflow, the venous outflow, and the management of the exocrine part of pancreas. The techniques of PTx vascularization in rats are based on two basic models; the microsuture and non-suture cuff techniques. Although more difficult than the cuff technique, microsuture technique is often the preferred method for vascularization. The purpose of this paper is to explain how to use of surgical techniques in the rat and dog pancreas transplantation model and also advantages and disadvantages of vascularization techniques.
