Turan, Nazli Istanbul Üniversitesi - Deneysel Tip Arastirma Enstitüsü - Moleküler Tip Anabilim Dali, Turkey , Ergen, Arzu Istanbul Üniversitesi - Deneysel Tip Arastirma Enstitüsü - Moleküler Tip Anabilim Dali, Turkey , Arikan, Soykan Istanbul Egitim ve Arastirma Hastanesi - Cerrahi Klinigi, Turkey , Yaylim, Ilhan Istanbul Üniversitesi - Deneysel Tip Arastirma Enstitüsü - Moleküler Tip Anabilim Dali, Turkey , Isbir, Turgay Yeditepe Üniversitesi - Tip Fakültesi - Tibbi Biyoloji Anabilim Dali, Turkey

The Role Of Mmp-3 And E-cadherin Polymorphisms In Breast Tumours

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Breast cancer and other malignities are caused by genetic alterations affecting cellular pathways participating in cell growth and development. Proto-oncogenes contain certain proteins which are impotant for normal cell growth and differentiation. If a mutation causes a damage by changing in the structure of the proto-oncogene, it eliminates the control of cell division in various ways by changing the structure of genes and gene products and malignity consists. Except the oncogenes, a second gene group called tumor suppresor genes are also important in formation of cancer. Cadherins are connected each other by homophilic binding and they play important roles in cell adhesion, ensuring that cells within tissues are bound together. Because of the irregular behavior of tumor cells to cell-cell relationship is corrupted. E-Cadherin (CDH-1) leads to the destruction of property of the cell mobility. In breast cancer issues, due to lost of E-Cadherin expression or disorder in phosphorylation of cytoplasmic part of E-Cadherin, epithelial cells gain mobility. And increase in their local spread is observed. The lack of E-cadherin is responsible for emergence of invasive phenotype and ductal carcinomas, leading to a poor prognosis. Matrix metalloproteinases (MMP) gene family, codes 9 or more endopeptidase against extracellular matrix macromolecules. It has been reported that increased MMP-3 expression in breast cancer is encouraging tumor prognosis. In this study, we aimed to investigate the effect of the E-Cadherin - 160 C/A and MMP-3 5A/6A gene polymorphisms in breast cancer. For this purpose, to determine relevant gene polimorphisms we used polymerase chain reaction-restriction fragment lenght polymorphism (PCR-RFLP) method. As a result of statistical consideration, we find out that carrying 6A allel of MMP-3 gene and CC genotype of E-Cadherin can play a role in disease progression and metastasis.

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MMP , 3 , E , Cadherin , Matrix Metalloproteinases , Polymorphism , Breast cancer

Deneysel Tıp Araştırma Enstitüsü Dergisidir

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