Author/Authors :
Ng, Ozden Hatirnaz Istanbul University - Institute for Experimental Medical Research - Genetics Department, Turkey , Can, Ismail Istanbul University - Institute for Experimental Medical Research - Genetics Department, Turkey , Firtma, Sinem Istanbul University - Institute for Experimental Medical Research - Genetics Department, Turkey , Erbilgin, Yucel Istanbul University - Institute for Experimental Medical Research - Genetics Department, Turkey , Ozbek, Ugur Istanbul University - Institute for Experimental Medical Research - Genetics Department, Turkey , Sayitoglu, Muge Istanbul University - Institute for Experimental Medical Research - Genetics Department, Turkey
Abstract :
8q24 region, localized in a TCF4 transcription factor consensusbinding sequence, harbors many independent risk polymorphisms and is to be associated to several cancers. In this study, we aimed to know if the allele specific regulation of TCF4 is applicable/admissible for chUdhood acute leukemia by genotyping 8q24 and investigating the expression of three main targets of TCF4 (c- MYC, LEF1, AXIN2). Allele frequencies of rs6983267 were not significantly different between the acute leukemia cases and the combined analysis of the frequencies (GG vs GT+TT or TT vs GT +GG) did not show a significant association but the GG genotype and mRNA expressions of target genes showed a correlation. Also in B- ALL patients, only c-MYC gene expression was increased. We also genotyped acute leukemia cell lines and cell line gene expressions did not show a consistent and significant correlation between the genotypes. To sum up our findings showed that, the frequency of the variation at 8q24 is not associated with ALL risk directly but it is clear that tissue specific differences and regulations may attend to TCF4 regulation and its targets.
NaturalLanguageKeyword :
leukemia , cancer , TCF4 , polymorphism
