مرکز منطقه ای اطلاع رساني علوم و فناوري - Antifungal resistance of candida isolates obtained from various specimens of intensive care unit patients

Abstract :

Objectives: In this study; we aimed to determine the identification of yeasts from the samples of the patients that come from Anesthesia Intensive Care Unit of Diyarbakır Education and Research Hospital and also we aimed to perform the antifungal susceptibility testing of yeasts. Materials and methods: Antifungal susceptibility test results of yeasts that isolated from 25 blood, 24 urine, 3 sputum and 3 peritoneal fluid samples of the patients that come from Anesthesia Intensive Care Unit to our laboratory during the period December 2009 - Septemberl/2010 were evaluated. The yeasts identified by germ tube test, cornmeal tween 80 and VITEC 2 Compact® (Biomerieux, France) yeast identification system. The antifungal susceptibility tests were performed for amphotericin B, flucytosine, fluconazole and voriconazole by using VITEC 2 Compact® (Biomerieux, France) system. Results: 56.36% of the yeasts were determined as C.albicans which was the most common yeast fol-lowed by; C.parapsilosis (30.9%), C.tropicalis (10.6%), C.tropicalis (5.45%), C.dubliniensis (3,63%), C.glabrata (1.81%) and C.guilliermondi (1.81%). According to the results of antifungal susceptibility tests, the resistance rate for fluconazole and variconazole were 1.81% and 3.63% respectively. However no resistance were detected against amphotericin B and flucytosine. Conclusions: Our results shows that C.albicans is the most common yeast isolated from the patients at intensive care unit in our hospital. Increased resistance to fluconazole which is frequently used for empirical treatment demostrates importance of antifungal susceptibility tests.