Karagenç, Nedim Pamukkale Üniversitesi - Tıp Fakültesi - Biyokimya AD, Turkey , Meydancı, Duygu Pamukkale Üniversitesi - Tıp Fakültesi - Biyokimya AD, Turkey , Küçüksayan, Hakan Pamukkale Üniversitesi - Tıp Fakültesi - Biyokimya AD, Turkey

Association of hypoxia inducible factor 1 (HIF-1) and paraoxonase enzyme in lung cancer cell lines

27212

Objective: In this study, it was aimed to investigate that PON2, in hypoxia and normoxia in relation to HIF-1α transcription factor that has a role in tumour angiogenesis. Hypoxia inducible factor 1 alpha (HIF-1α) is a transcription factor which is sensitive to hypoxia, causes initiation of angiogenesis and metastasis by providing transcription of numerous genes. As well as hypoxia several inflammatory agents such as lipopolysaccharide (LPS) can regulate the expression of HIF-1α as well as PON2. Oxidative stress is known to have role in cancer. Paraoxonase 2 which is one of the members of paraoxonase family serves as intracellular anti-oxidant.Methods: H1299, A549 ve PC14 non-small cell lung carcinoma cell lines used in this study. Cells were cultured under hypoxia and normoxia conditions with LPS stimulation. HIF-1α and PON2 mRNA expression levels measured by real-time PCR. Western blot studies were performed for protein expression.Results: In this study, it was observed that LPS treatment stimulates HIF-1α expression which increases PON2 expression in NSCLC cell line in under hxpoxia conditions.Conclusion: This study shows that PON2 is regulated by HIF-1α in hypoxia and inflammation. The relationship between hypoxia and inflammation and oxidative status of cells requires further studies.

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HIF , 1α , PON2 , LPS , Hypoxia

Dicle Medical Journal

367