dokumacioglu, eda artvin coruh university - faculty of healthy sciences - department of nutrition and dietetics, Turkey , iskender, hatice artvin coruh university - faculty of healthy sciences - department of nutrition and dietetics, Turkey , aktas, mustafa sinan ataturk university - faculty of veterinary medicine - department of internal medicine, Turkey , hanedan, basak ataturk university - faculty of veterinary medicine - department of internal medicine, Turkey , dokumacioglu, ali hopa government hospital - department of medical biochemistry, Turkey , sen, tugba mazlum karadeniz teknik university - faculty of medicine - department of medical biochemistry, Turkey , saral, sinan recep tayyip erdoğan university - school of physical therapy and rehabilitation - department of physiotherapy and rehabilitation, Turkey

The effect of sulforaphane on the levels of serum cystatin-c in acetaminophen-induced nephrotoxicity in rats

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Objective: The exposure of living creatures to drugs and chemicals often results in toxicity of liver and kidney. Drugs constitute an important and big part of the community and hospital-acquired kidney diseases. In this study, we investigated the effect of sulforaphane (SFN) on the levels of cystatin-C and lipid peroxidation on acetaminophen (APAP)- induced nephrotoxicity in rats.Methods: Thirty-six Sprague-Dawley rats were separated equally into four experimental groups: control group, SFN group, APAP group, and APAP + SFN group. In the experimental treatment groups APAP was administered oral gavage at 1 g/kg 3 h after SFN treatment in last day and, in the APAP + SFN group, SFN was administered oral gavage at a dose of 500 μg/kg exactly for three days. Rats were euthanized and sacrificed 24 h after APAP administration. Results: APAP administration showed to significant increase in serum BUN, creatinine, urea and LDH concentrations as compared to the control datas indicating the induction of severe nephrotoxicity (p 0.001). SFN treatment significantly decreased the cystatin-C levels and lipid peroxidation compared to APAP group (p 0.05).Conclusion: The present study demonstrate that the attachment of SFN to the nephrotoxicity treatment protocol will be beneficial and further studies should be conducted for cystatin C which plays an important role in kidney toxicity and disease to be routinized as a biomarker.

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Acetaminophen , Cystatin , C , Lipid peroxidation , Nephrotoxicity

Dicle Medical Journal

389