Author/Authors
Hanoğlu, Şakire Afyon Kocatepe University - Faculty of Science and Literature - Biology Department, Turkey , Korcan, S. Elif Afyon Kocatepe University - Faculty of Science and Literature - Biology Department, Turkey , Erdoğmuş, S. Feyza AfyonKocatepe University - Technical Vocational School of Higher Education of Bayat - Department of Laboratory and Veterinary Health, Turkey , Konuk, Muhsin Uskudar University - Faculty of Engineering and Natural Sciences - Department of Molecular Biology and Genetics, Turkey
Title Of Article
Comparison of 16S-ITS rDNA RFLP Profiles of Bacillus sp. Isolated from Milk and Different Water Sources
شماره ركورد
27597
Abstract
The aim of this research was the isolation of thermophilic Bacillus sp. from different sources and grouping by 16S-ITS rDNA RFLP. Seventy-four thermophilic strains were isolated from various environmental samples. Since fifteen isolated strains were Gram (+), endospore forming rods, they were identified as Bacillus sp. These strains were screened for the existence of five extracellular enzyme activities. These were, lipases, amylases, proteases, xylanases, cellulases. 16S-ITS rDNA RFLP profiles were produced by using two restriction endonucleases Taq I and Hae III. The isolated strains were clustered into eigth groups by Taq I restriction profiles of 16S-ITS rDNA while seven groups were obtained by Hae III digestion profiles.
From Page
29
NaturalLanguageKeyword
Bacillus sp. , Extracellular enzyme , 16S , ITS rDNA , RFLP
JournalTitle
Afyon Kocatepe University Journal Of Science and Engineering
To Page
38
JournalTitle
Afyon Kocatepe University Journal Of Science and Engineering
Link To Document