Hanoğlu, Şakire Afyon Kocatepe University - Faculty of Science and Literature - Biology Department, Turkey , Korcan, S. Elif Afyon Kocatepe University - Faculty of Science and Literature - Biology Department, Turkey , Erdoğmuş, S. Feyza AfyonKocatepe University - Technical Vocational School of Higher Education of Bayat - Department of Laboratory and Veterinary Health, Turkey , Konuk, Muhsin Uskudar University - Faculty of Engineering and Natural Sciences - Department of Molecular Biology and Genetics, Turkey

Comparison of 16S-ITS rDNA RFLP Profiles of Bacillus sp. Isolated from Milk and Different Water Sources

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The aim of this research was the isolation of thermophilic Bacillus sp. from different sources and grouping by 16S-ITS rDNA RFLP. Seventy-four thermophilic strains were isolated from various environmental samples. Since fifteen isolated strains were Gram (+), endospore forming rods, they were identified as Bacillus sp. These strains were screened for the existence of five extracellular enzyme activities. These were, lipases, amylases, proteases, xylanases, cellulases. 16S-ITS rDNA RFLP profiles were produced by using two restriction endonucleases Taq I and Hae III. The isolated strains were clustered into eigth groups by Taq I restriction profiles of 16S-ITS rDNA while seven groups were obtained by Hae III digestion profiles.

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Bacillus sp. , Extracellular enzyme , 16S , ITS rDNA , RFLP

Afyon Kocatepe University Journal Of Science an‎d Engineering

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