HALILOGLU, Kamil Ataturk University - Faculty of Agriculture - Department of Field Crops, TURKEY , BEZIRGANOĞLU, İsmail Eastern Anatolia Agricultural Research Institute, TURKEY , AYDIN, Murat Ataturk University - Faculty of Agriculture - Department of Field Crops, TURKEY , TOSUN, Metin Ataturk University - Faculty of Agriculture - Department of Field Crops, TURKEY , ŞAHIN, Esin Ataturk University - Faculty of Agriculture - Department of Field Crops, TURKEY

Response of Current Winter Wheat Cultivars Grown in Turkey to Immature Embryo Culture

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Selecting the explant genotypes is crucial step in in-vitro culture and Agrobacterium-mediated transformation system due to its host range specificity. Poor tissue culture performance is restricting the number of wheat genotypes that can be stably transformed. Response of fourteen cultivars of winter wheat (Triticum aestivum L.) to immature embryo culture, callus production and plant regeneration was evaluated. A significant (p 0.05) genotypic difference in callus induction frequency was observed ranging between 45% (cv. Sürak) and 99.54% (cv. Bobwhite) of the cultured explants. Plant regeneration through somatic embryogenesis was observed from calli of all 15 cultivars after a 4-week callus induction period. Genotypes with the highest number of plantles per cultured embryo were Sivas-111/33 (8.66), Kıraç-66 (7.36), Haymana 79 (6.77) and Conkesme (6.56). Four winter wheat genotypes, ‘Sivas-111/33, Kirac-66, Haymana 79, and Conkesme’, have been selected for initial Agrobacterium-mediated transformation experiments due to their high regeneration potentials.

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Winter wheat , callus formation , plant regeneration , immature embryos

Journal Of Agricultural Faculty Of Atatürk University

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