Author/Authors :
Ödemiş, Zeliha Beşikçi Cumhuriyet Üniversitesi - Tıp Fakültesi - Tıbbi Farmakoloji Anabilim Dalı, Turkey , Temiz, Tijen Kaya Cumhuriyet Üniversitesi - Tıp Fakültesi - Tıbbi Farmakoloji Anabilim Dalı, Turkey , Balcı, Ezgi Cumhuriyet Üniversitesi - Tıp Fakültesi - Tıbbi Farmakoloji Anabilim Dalı, Turkey , Parlak, Mesut Cumhuriyet Üniversitesi - Tıp Fakültesi - Tıbbi Farmakoloji Anabilim Dalı, Turkey , Altun, Ahmet Cumhuriyet University - School of Medicine - Tıbbi Farmakoloji Anabilim Dalı, Turkey
Abstract :
Aim. The purpose of this study is to compare the effects of Simvastatin and Mevastatin, commonly used statins in clinic practice, on angiogenesis in chorioallontioc membrane models by using fertilized chicken eggs. Methods. In this study, Ross 308, fertilized chicken eggs were used. The fertilized chicken eggs were incubated in horizontal position with environmental conditions of 37.5 °C temperature and 80% relative humidity. On the fifth day of the incubation period, 5 ml of albumen was taken through the eggshell with a syringe and a shell piece of 2-3 cm in diameter was removed from the contrary side of the eggs. The windows on the egg shells were sealed with gelatin and thereafter, the eggs were incubated for 72 more hours to have chorioallontioc membrane models reaching 2 cm in diameter. The pellets containing active substrate were placed on the chorioallantoic membrane of each egg. After drug administration (bevacizumab, Simvastatin and Mevastatin; 10-4 M, 10-5 M ve 10-6 M concentration), the eggs were incubated for 24 hours again. Under a stereoscopic microscope, vascular structure around the pellets was evaluated using a scoring system of Bürgermeister et al. Results. Drug-free agar discs were used as negative control in our study. Angiogenesis was not inhibited in any negative control eggs and anti-angiogenic effect score was found as “0”.Bevacizumab was used as positive control. In 10-4 M, 10-5 M and 10-6 M concentrations, antiangiogenic score of Bevacizumab were found 1,58, 1,55 and 1,00, respectively. For 10-4 M, 10-5 M and 10-6 M concentrations of Simvastatin and Mevastatin, antiangiogenic scores were found as 0.93, 0.66, 0.53 and 1.0, 0.80, 0.66, respectively. Both Mevastatin and Simvastatin were found to have antiangiogenic effects in each three concentrations. There wasn‟t a significant difference between antiangiogenic effects of these two drugs in 10-4 M. But when lower concentrations were compared it is found that Mevastatin had significantly higher antiangiogenic effect than Simvastatin at 10-5 M and 10-6 M concentrations. Conclusion. In conclusion, Simvastatin and Mevastatin are statins which shows antiangiogenic effect in chorioallontioc membrane models. Besides they can the lipid-lowing effects of statins they can also provide. Protection against plaque rupture related serious complication by the intraplaque antiangiogenesis effect
NaturalLanguageKeyword :
Simvastatin , mevastatin , chorioallontioc membrane models , angiogenesis
