Author/Authors :
GÜNGÖR, Şükrü Mehmet Akif Ersoy University - Faculty of Veterinary Medicine - Department of Reproduction and Artificial Insemination, TURKEY , AKSOY, Adil Republic of Turkey, Prime Ministry, TURKEY , YENİ, Deniz Afyon Kocatepe University - Faculty of Veterinary Medicine - Department of Reproduction and Artificial Insemination, TURKEY , AVDATEK, Fatih Afyon Kocatepe University - Faculty of Veterinary Medicine - Department of Reproduction and Artificial Insemination, TURKEY , ÖZTÜRK, Caner Aksaray University - Faculty of Veterinary Medicine - Department of Reproduction and Artificial Insemination, TURKEY , BOZKURT ATAMAN, Mehmet Selcuk University - Faculty of Veterinary Medicine - Department of Reproduction and Artificial Insemination, TURKEY , COYAN, Kenan Pamukkale University - Faculty of Medicine - Department of Histology and Embryology, TURKEY , BUCAK, Mustafa Numan Selcuk University - Faculty of Veterinary Medicine - Department of Reproduction and Artificial Insemination, TURKEY , BAŞPINAR, Nuri Selcuk University - Faculty of Veterinary Medicine - Department of Biochemistry, TURKEY , PEKER AKALIN, Pınar Mustafa Kemal University - Faculty of Veterinary Medicine - Department of Biochemistry, TURKEY
Abstract :
ABSTRACTThe present study was conducted to examine the protective roles of cysteamine, trehalose, alpha-lipoic acid and combinationsof these antioxidants on post-thawed bull sperm and oxidative stress parameters. Five healthy Holstein bull (3-4 years old) were used. Eight ejaculates for each bull were collected and pooled. Pooled ejaculate, splitted into seven equal aliquots and diluted at 37 °C with base extenders containing cysteamine 2 mM, trehalose 50 mM, alpha-lipoic acid (ALA) 1 mM, cysteamine 2 mM + trehalose 50 mM, ALA 1 mM + trehalose 50 mM, cysteamine 2 mM + ALA 1 mM and no antioxidant (control), was cooled to 5 °C and then frozen. Frozen straws were thawed in a water bath for evaluation. The combination of cysteamine 2 mM and ALA 1 mM of the semen extender improved the percentages of post-thawed subjective motility (68 ± 2.7%), and progressive motility (42.9 ± 4.7%), compared with the controls (61 ± 4.2% and 37.5 ± 8%, respectively, non- significantly, P 0.05). The supplementation of the semen extender with combination of cysteamine 2 mM and ALA 1 mM produced a higher acrosome integrity and mitochondrial activity (52.02 ± 6.4% and 32 ± 4.1%, respectively), compared with the controls (30.5 ± 1.7 and 14.02 ± 3.5% respectively, P 0.05). Combination of cysteamine and ALA antioxidants in semen extenders provided the benefit in terms of sperm motilities, acrosome integrity and mitochondrial activity on frozen-thawed bull sperm
NaturalLanguageKeyword :
Alpha Lipoic Acid , Bull Sperm , Cysteamine , Fluorescent Staining , Trehalose
