مرکز منطقه ای اطلاع رساني علوم و فناوري - Analysis of p-53exon 8 gene mutations in 3-methylcholanthrene and butylated hydroxytoluene-induced rat lung tissues

Abstract :

Aim. In this study we aimed to detect the carcinogenic effects on lungs of rat and, also the mutation effects on P53 ekzon 8 gene of Butylated hydroxytoluene (BHT) and BHT together with 3-Metilkolantren (3-MC) which is a carcinogenic chemical substance. Method. In this study, 52 male rats were used. 15 rats in the first group (D1) are administered 3-MC i.p 40 mg/kg per week during 6 weeks. 15 rats in the second group (D2) were given a single dose of 3-MC i.p. 40 mg/kg and subseguently BHT 200 mg/kg of body weight per week for 6 weeks. 12 rats in the third group (D3) were exposed to BHT 200 mg/kg of body weight on weekly basis for 6 weeks. 10 rats in Control (c) group were only given corn oil in which both 3-MC and BHT are soluable, for 6 weeks receiving 100 l for each shot. The rats were killed with cervical dislocation at the end of 26 weeks. Lung tisues were evaluated by scintigraphic, radiologic and morphologic methods. DNA isolation was carried out tissues of the lungs followed by Polymerase chain reaction (PCR) amplification for exon 8 of p53 gene. Then, in the PCR products mutational analysis was carried out using Single-Strand Conformation Polimorphism (SSCP) technique. Results. Cancer development has not been detected in lung tissues of the rats that were exposed to 3-MC and BHT. However, it has been found that subcutaneous soft tissue sarcomas were detected at the application site of carcinogens. In p53 gene exon 8 screening, there is no mutation were technique found in the lung tissues of all rats. Conclusion. Although we could not detect any cancer formation in our rats, we think that using appropriate dose and timing of 3-MC and BHT in especially cancer sensitive rats is convenient.