Investigation of the effects of drugs effective on PI3K-AKT signaling pathway in colorectal cancer alone and in combination

33404

Aim. This study was designed to determine the agent and/or possible agent combinations that may be effective in the treatment of colorectal cancer. For this reason the single and combined antiproliferative effects of NVPBEZ-235, API-1, LY 294002 and PP242 which target PI3K/AKT pathway well known to have an important role in pathophysiological mechanisms of colorectal cancer were investigated. We investigated whether NVPBEZ-235, API-1 and PP242 have antiproliferative effects which have not been studied on this cancer type and also compared the effects with LY294002 known to have antiproliferative effect on colorectal cancer. Method. DLD-1 cell line was used as colorectal cells. Real time cell analysis (xCELLigence system) was used to determine the effects of the agents in colorectal cell proliferation. Results. When the agents were applied alone, PI3K/mTOR dual inhibitor NVP-BEZ had the strongest cytotoxic effect and PI3K inhibitor LY294002 had the lowest cytotoxic effect. None of the combinations were not superior compared to alone applications. Conclusion. This study indicates that when PI3K/AKT pathway is targeted, instead of combined treatment, targeted specific treatment with a single agent having higher affinity and effectiveness would be more appropriate. Also among the agents effecting PI3K/AKT pathway especially, NVP-BEZ for targeted therapy can be alternative to conventional treatments.

167

Colorectal cancer , PI3K , AKT pathway , NVPBEZ , 235 , API , 1 , LY 294002 , PP242

Cumhuriyet Medical Journal

177

Cumhuriyet Medical Journal