SERDAR, Umit Ondokuz Mayis University - Agricultural Faculty - Horticulture Department, Turkey , MERCAN, Levent Ondokuz Mayis University - Agricultural Faculty - Agricultural Biotechnology Department, Turkey , OKUMUS, Ahmet Ondokuz Mayis University - Agricultural Faculty - Agricultural Biotechnology Department, Turkey , SOYLU, Arif Uludag University - Agricultural Faculty - Horticulture Department, Turkey

MORPHOLOGICAL AND MOLECULAR COMPARISON OF SELECTED CHESTNUT (Castanea sativa Mill.) GENOTYPES FROM BLACK SEA REGION OF TURKEY

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Here we report phenotypic and genotypic differences among 14 Turkish chestnut genotypes. The genotypes were analyzed both genetically and for 30 morphological criteria comprising 12 qualitative and 18 quantitative characteristics. The phylogenetic relationships were determined between different chestnut genotypes selected from Sinop, Samsun, Artvin and Bartin provinces of Black Sea Region in Turkey. Morphological criteria were investigated biometrically using multivariate analysis. Ten morphological criteria were found to be effective for discrimination between the genotypes. Five morphological criteria accounts for 64.1% of the variability. These morphological criteria were the ratio of length of teeth to width of teeth, the ratio of length of hilum to length of fruit, cross section of leaves, the ratio of height of fruit to length of fruit and peeling of testa. The discrimination of morphological criteria was shown using cluster analysis, which created four main groups. Dice’s coefficient was used to evaluate the genetic similarity by RAPD analysis, which created three main groups. The UPGMA method was used for the determination of phylogenetic trees. The genetic and morphological dendrograms were compared using the Mantel test, which gave a correlation of r=-0.33. The discrimination of morphological criteria was shown using cluster analysis, which created four main groups. Dice’s coefficient was used to evaluate the genetic similarity by RAPD analysis, which created three main groups. The UPGMA method was used for the determination of phylogenetic trees. The genetic and morphological dendrograms were compared using the Mantel test, which gave a correlation of r=-0.33. This study illustrates that the selected chestnut genotypes might be valuable genetic resources for future chestnut breeding programs.

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Genetic similarity , morphological criteria , phylogenetic tree , RAPD

Anadolu Journal Of Agricultural Sciences

62