Author/Authors :
Akdemir, Hülya Gebze Teknik Üniversitesi - Temel Bilimler Fakültesi - Moleküler Biyoloji ve Genetik Bölümü, Turkey , Akbulak, Tuğçe Gebze Teknik Üniversitesi - Temel Bilimler Fakültesi - Moleküler Biyoloji ve Genetik Bölümü, Turkey , Süzerer, Veysel Gebze Teknik Üniversitesi - Temel Bilimler Fakültesi - Moleküler Biyoloji ve Genetik Bölümü, Turkey , Süzerer, Veysel Bingöl Üniversitesi - Tıbbi Hizmetler ve Teknikler Bölümü, Turkey , Kayıhan, Doğa Gebze Teknik Üniversitesi - Temel Bilimler Fakültesi - Moleküler Biyoloji ve Genetik Bölümü, Turkey , Koç, İbrahim Gebze Teknik Üniversitesi - Temel Bilimler Fakültesi - Moleküler Biyoloji ve Genetik Bölümü, Turkey , Çiftçi, Yelda Özden Gebze Teknik Üniversitesi - Temel Bilimler Fakültesi, Biyoteknoloji Enstitüsü - Moleküler Biyoloji ve Genetik Bölümü, Turkey
Abstract :
There is an increasing demand to ornamental plants in the World. However, problems faced with the rooting of cuttings in the propagation of ornamental fraser photinia plants. Plant tissue culture techniques are used in several plant species to overcome such difficulties of vegetative production. Therefore, the aim of this study concerns the identification of optimum plant growth regulator content in semi-solid culture for the micropropagation of economically important fraser photinia. Thus, different cytokinin types [BA (6-benzyladenine), KIN (Kinetin); TDZ (Thidiazuron); 2-iP (2 –isopentyl adenine)] and amounts (0, 1, 2, 4 mg l^-1) together with various carbon sources (sucrose and glycose) and amounts (0, 15, 30 g l^-1) were assessed in semi-solid medium. The highest proliferation rate (100%) and the maximum number of shoots proliferated per explant (3.7) were obtained in shoot tips of fraser photinia on semi-solid MS medium supplemented with 2 mg l^-1 BA and 30 g l^-1 sucrose. The highest rooting (40%) was achieved in proliferated shoots on semi-solid medium supplemented with 4 mg l^-1 IBA for 1 week followed by transfer of the microshoots to auxin-free medium. Rooted shoots were then successfully acclimatized to in vivo conditions.
