مرکز منطقه ای اطلاع رساني علوم و فناوري - Determination of prevalence and genetic diversity of plum pox virus (sharka) in peach orchards in Bursa

Abstract :

Plum pox virus (PPV) is the most important viral disease of stone fruit species. Recent nucleotide Plum pox virus (PPV) is the most important viral disease of stone fruit species. Recent nucleotide sequencing studies have revealed that PPV is prevalent and exhibits high genetic diversity in residential gardens of several provinces in Turkey. Trees in residential gardens have been found to be infected by Turkish type PPV-D and PPV-T while trees in professional orchards in several cities have been found to be affected by the European strain PPV-M. The province Bursa is main peach producer region in Turkey but moreover Bursa hosts big nursery industry and is the distribution center of seedlings imported from European countries. In this research, surveys were made in the orchards of Bursa and surrounding regions, and comparisons were made in order to investigate genetic relationships among the isolates of Bursa and other PPV isolates determined in Turkey as well as European PPV isolates. A total of 118 samples from 50 orchards were examined by DASI-ELISA and RT-PCR methods. Of the all, 64 samples from 32 orchards were found to be infected with PPV. The P3-6K1 gene region with 664 nt length and the CP-3’UTR with 970 nt of the 64 isolates were sequenced and analyzed along with 200 sequence records in the GenBank database. Fifty-six isolates were identified as European type PPV-M. Eighth isolates from two plums orchard in Bursa were identified as PPV-D and grouped with Turkish type PPV-D. These results indicate that Bursa-Turkey seedlings traffic pathway seems to be the source of PPV-M infection in the orchards in the country.