TAŞDEMİR, Umut Ministry of Food, Agriculture and Livestock - Lalahan Livestock Central Research Institute, Turkey , SATILMIŞ, Muharrem Ministry of Food, Agriculture and Livestock - Lalahan Livestock Central Research Institute, Turkey , KARAŞAHİN, Tahir Ministry of Food, Agriculture and Livestock - Lalahan Livestock Central Research Institute, Turkey , KIZIL, Sedat Hamdi Ministry of Food, Agriculture and Livestock - Lalahan Livestock Central Research Institute, Turkey , KAYMAZ, Mustafa Ankara Üniversitesi - Faculty of Veterinary Medicine - Department of Obstetrics and Gynecology, Turkey , IMAI, Kei National Livestock Breeding Center, Japan

The effect of single epidural plus intramusculer injection of FSH on superovulatory response in Anatolian Black cow

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The present study was designed to determine whether a single epidural injection plus intramuscular (im) injection of FSH in order to reduce the number of injection treatments required to induce supurovulatory response, as well as to determine the effects of this treatment on Anatolian Black cow (Bos Taurus). The results will be compared to those obtained by administration of twice daily injections of FSH. Twenty-one Anatolian Black cows were used in this study. Anatolian Black cows received Cue-mate containing 1.56 g progesterone. The cows were divided into three groups (D1, D2 and E) based on the dose and administration route of FSH. In group D1 a total of 490 IU FSH and in group D2 a total of 700 IU FSH was given intramuscularly in decreasing doses for twice daily for 4 days. In both groups superovulatory treatments were initiated on day 7 of the cycle. In group E, FSH was given 175 IU epidural plus 175 IU im injection on day 7 of the cycle. On the 9th day of the cycle, the Cue-mate was removed and the cowsreceived 500 μg prostaglandinF2α in all goups. The cows were artificially inseminated (AI) using semen obtained from nativeAnatolian Black bulls twice; in the evening of day 11 and in the morning of day 12 with 12 hours intervals. Superovulated cows werenon-surgically flushed 7 days after AI. Embryos were morphologically evaluated and classified according to the criteria recommended by the IETS. No significant differences were observed in the mean number of Corpus luteum (CL) among groups which were 3.7 ± 0.56 in D1; 3.4 ± 0.55 in D2 and 6.0 ± 1.14 in E . There was no difference in transferable embryo yield among groups (10/42, 23.8% in D1; 14/32, 43.8% in D2 and 19/82, 23.2% in E). Untransferable embryo yield was not also significantly different among the groups (32/42, 76.2% in D1; 18/32, 56.2% in D2 and 63/82, 76.8% in E). The UFO rate was similar in all groups (17/77, 22.1% in D1; 13/71, 18.3% in D2 and 28/132, 21.2% in E). Recovery rate was found to be significant among groups (59/77, 76.6% in D1; 45/71, 63.4% in D2 and 110/132, 83.3% in E). To conclude, a single epidural injection plus an im injection of FSH application can be obtained acceptable results compared to twice daily injection of FSH for superstimulatory response. However transferable embryo rate of Anatolian Black cow was found to be low in both single epidural injection plus im injection and twice daily FSH applications. Further studies are required to obtain more concrete results on increasing transferable embryo yields in different breeds.

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Cow , embryo , epidural injection , FSH , superovulation

Veterinary Journal of Ankara University

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