Author/Authors :
Arıcı, Mürsel Ankara Üniversitesi - Fen Fakültesi - Kimya Bölümü, Turkey , Nazır, Hasan Ankara Üniversitesi - Fen Fakültesi - Kimya Bölümü, Turkey
Title Of Article :
Synthesis and Investigation of DNA Effect of [N,N -bis (salicylidene)-1,3-propanediaminato]nickel(II) Complex
Abstract :
In this study, N,N -bis(salicylidene)-1,3-propanediaminato]nickel(II) (NiL) complex was synthesized from reaction of bis(salicylidene)-1,3-propanediamine (H2L) with nickel(II) chloride hexahydrate in ethanolic medium. The interaction of synthesized NiL complex with pBR322 plasmid DNA was investigated by ultraviolet absorption spectroscopy (UV), cyclic voltammetry (CV) and agarose jel electrophoresis. It was found that the absorption spectrum of NiL complex in the presence of plasmid DNA showed hyperchromism and its peak current was significantly decreased at recorded voltammogram. Effect of NiL complex on DNA in the absence of reductant agents showed by ajarose jel electrophoresis. Moreover, the geometry of the lowest-energy structure of NiL complex calculated by Hyperchem® program gave a good result to be associated with partial intercalation and electrostatic binding modes of the DNA.
NaturalLanguageKeyword :
Ni(II) complex , plasmid DNA cleavage , partial intercalation , electrostatic interaction
JournalTitle :
Süleyman Demirel University Faculty of Arts and Science Journal of Science
