Abstract :
The effect of 4 different types of explants (proximal part, distal part, whole cotyledon, and hypocotyl) and 36 different hormone combinations on callus proliferation and shoot regeneration were evaluated in ornamental gourd (Cucurbita pepo var. ovifera). The explants were taken from 8-day-old seedling cultured on Murashige and Skoog (MS) media containing different doses of 2,4-dichlorophenoxyacetic acid (2,4-D) (2.0, 2.5, and 3.0 mg l^-1) for callus induction. Generated calli were cultured in combinations of 6-benzylaminopurine (BAP) (0, 0.5, 1.0 and 2.0 mg l^-1) with different doses of α-naphthaleneacetic acid (NAA) (0, 0.1 and 0.5 mg l^-1), indole-3-acetic acid (IAA) (0, 0.1 and 0.5 mg l^-1) and kinetin (0, 1.0 and 2.0 mg l^-1) in MS media. The maximum callus induction ratio (%) in all types of explants was obtained from MS media supplemented with 2.0 mg l^-1 2,4-D. BAP (1.0 and 2.0 mg l^-1) combinations with NAA (0.1 and 0.5 mg l^-1) were more successful in hypocotyl and proximal types of explants in terms of callus proliferation (%). The highest mean number of shoots per explant were obtained from 0.5 mg l^-1 BAP + 1.0 mg l^-1 kinetin in hypocotyl (1.8) and proximal explants (2.6).
NaturalLanguageKeyword :
Callus , Cucurbita pepo var. ovifera , Organogenesis , Regeneration
