Author/Authors :
mokhtari, w. institute of agronomy and veterinary sciences - plant production, protection and biotechnology department, Morocco , chtaina, n. institute of agronomy and veterinary sciences - plant production, protection and biotechnology department, Morocco , halmschlager, e. university of natural resources and life science - institute of forest entomology, forest pathology and forest protection (ifff), Austria , volgmayr, h. university of vienna - faculty centre of biodiversity - department of systematic and evolutionary botany, Austria , stauffer, c. university of natural resources and life science - institute of forest entomology, forest pathology and forest protection (ifff), Austria , jaklitsch, w. university of vienna - faculty centre of biodiversity - department of systematic and evolutionary botany, Austria
Title Of Article :
Potential antagonism of some Trichoderma strains isolated from Moroccan soil against three phytopathogenic fungi of great economic importance
Abstract :
In this study, 17 Trichoderma spp. strains were isolated from different soils (Argan forests and crop fields) in Morocco. Purified monospore cultures were identified using molecular methods and tested for their potential antagonism against three phytopathogenic fungi (Fusarium oxyxporum, verticillium dahlia and rhizoctonia solani). After DNA extraction, translation elongation factor (tef1) was amplified in extracts of 17 strains, sequenced and compared with their ex-types. As a result, three species were identified among the strains, which clustered in two different subclades of Trichoderma: the species T. afroharzianum, and T. guizhouense belong to Harzianum clade and T. reseei species belongs to Longibrachiatum clade. Dual culture plate assay was conducted confronting 17 Trichoderma isolates against Fusarium oxysporum, Verticillium dahlia and Rhizoctonia solani. In vitro, Trichoderma isolates showed effective antagonistic activity by decreasing mycelium growth of soil borne pathogens. Trichoderma afroharzianum showed the highest Percentage of Inhibition of Radial Growth (PRIG %). The highest PRIG% = 98% is when 8A2.3 isolate confront R. solani and the lowest PRIG% = 67% is when T9i10 confront F. oxysporum. On the other hand, T9i12, which is reesei species, significantly inhibit mycelium growth of the three pathogens.
NaturalLanguageKeyword :
Trichoderma spp , Fusarium oxysporum , Verticillium dahlia , Rhizoctonia solani , antagonistic fungi
JournalTitle :
Revue Marocaine des Sciences Agronomiques et Vétérinaires
