Author/Authors :
YARAŞ, Ali Bartın University - Engineering Faculty - Deparment of Metallurgy and Materials Engineering, Turkey , SELEN, Veyis Fırat University - Engineering Faculty - Department of Chemistry Engineering, Turkey , ÖZER, Dursun Fırat University - Engineering Faculty - Department of Chemistry Engineering, Turkey
Title Of Article :
SYNERGISTIC EFFECTS OF AGRO-INDUSTRIAL WASTES ON ALPHA AMYLASE PRODUCTION BY BACILLUS AMYLOLIQUEFACIENS IN SEMI SOLID SUBSTRATE FERMENTATION
Abstract :
This study concerns with the combinations of soybean meal (SM), wheat bran (WB) and whey were utilized as the substrates for α-amylase production by Bacillus amyloliquefaciens NRRL B-645. As a result of the experiments, the highest α-amylase activity (4257 U/ml) was obtained containing SM 20 g/l, WB 5 g/l, whey 5% (v/v), peptone 1 g/l, yeast extract (YE) 0.5 g/l and (NH4)2SO4 1 g/l at 33 °C and 150 rpm for 48 h. This innovative process for the α-amylase production can be extended to different enzymes using various agro-industrial wastes.
NaturalLanguageKeyword :
Alpha amylase , Bacillus amyloliquefaciens NRRL B , 645 , Agro , industrial wastes , Semi solid substrate fermentation
JournalTitle :
Pamukkale University Journal Of Engineering Sciences
