UTILIZATION OF ANTHER CULTURE FOR SCREENING OF DOUBLED HAPLOID LINES IN SOME TURKISH BREAD WHEAT HYBRIDS

41986

In the current research, it is aimed to investigate the doubled haploid (DH) productivity of 30 F2 derived wheat crosses constructed after hybridization of two Turkish bread wheat donors with different bread wheat genotypes, Sonmez01 and Es26. Especially, donor parents carrying out high yield performance and disease resistance selected from new developed bread wheat genotypes. Overall plant material sowed to the Transitional Zone Agricultural Research Institute field on 2010- 2011 season, and an average of 40 spikes at suitable development stage harvested from each group. Under anther culture conditions, MN6 used as initial culture media and modified 190-II choosed as regeneration media. Callus obtained from two thirds of F2 hybrids and they generated 16 albino plantlets. Extended ratios of callus production ranged between 3.25% and 50.5%. However green plantlet regeneration percentage calculated as 6.25% only in one hybrid, seven hybrid wheat samples generated only 0.25% green plantlet. According to overall results, five new DH wheat formed from different F2 hybrids derived from Sonmez01, while there were any dihaploid plants observed in F2 hybrids derived from Es26. In the sum, genome and phenome based evaluation of these F2 derived five doubled haploid bread wheat hybrids can help to describe an extra population source for next generation breeding platforms that they designed to extract new resistant crops against to environmental stresses. Also, modified anther culture method used in this study might be employed for screening of other bread wheat cultivars, lines and registered genotypes in the future DH wheat population construction.

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Anther culture , Albino , Bread wheat , Callus , Doubled haploid , Hybrid

Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology

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Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology