Author/Authors
poyraz, ismail bilecik seyh edebali university - faculty of art science - department of molecular biology and genetic, Turkey , gümüş, nurcan bilecik seyh edebali university - faculty of art science - department of molecular biology and genetic, Turkey
Title Of Article
COMPARISON OF RESISTANCE RATES AND DETECTION OF FIVE RESISTANCE GENES (BTS) IN TEN LOCAL WHEAT VARIETIES AGAINST COMMON BUNT DISEASE
شماره ركورد
41996
Abstract
Common bunt caused by Tilletia sp. is a very destructive and dangerous seed-borne fungal disease and may cause serious economic losses in worldwide. Wheat cultivars carrying resistance genes are used as an alternative fight method instead of chemical fungicides against common bunt disease. These resistance genes in wheat are called as bt genes. Until today, a few number of bt genes has been detected in wheat by using various molecular markers. However, development of more molecular markers for detection of all bt genes in wheat is required. In this study, detection of five bt genes called as bt-5, bt-8, bt-10, bt-11 and bt-12 was carried in ten registered local wheat varieties (Sertak 52, Bolal 2973, Demir, Kutluk, Harmankaya 99, Pehlivan, Tosun Bey, 4-11, Sönmez 01, Bezostaja-1) using PCR-based molecular markers, microsatellite and RAPD. PCR-amplified fragments were separated on 1.3% agarose gel. The obtained DNA bands were scored as present or absent for detection of bt genes. For comparison, the virulence rates of five Tilletia foetida (syn. leaves) isolates against ten wheat varieties were obtained from our field results. We observed that wheat varieties Kutluk and 4-11 carrying bt-10 and bt11 genes are more resistant to disease in field.
From Page
37
NaturalLanguageKeyword
Common bunt , Wheat , Bt resistance genes , Molecular markers
JournalTitle
Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology
To Page
45
JournalTitle
Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology
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