Evaluation of the Effects of Different Temperatures on Blood Cells’ Morphology and Life Time

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Objective: The aim of this study is to evaluate the effects of heat changes in blood tissue on blood cells’ morphology and apoptotic cell death. Material and Methods: The blood samples taken from quite healthy 6 women and 6 men from 18 to 25 years old have been used. These samples were assembled into 7 subgroups within itself and by increasing the temperature 2^oC per hour, all the samples’ heat was raised from 37^oC to 43^oC. During the heating, some part of the sample that was heated to 39^oC was cooled to 37^oC after an hour; this same process was applied on the samples that were heated to 41^oC and 43^oC. At the end of incubations 2 smears was prepared and stained with Giemsa and TUNEL based kit. Also, all of blood samples’ content was counted and biochemically, HSP70 titer was measured. Results: It was confirmed that high temperature induced apoptotic cellular death rate in leukocytes. It was also seen that in the groups with decreasing the death of the cells increased. Besides, there were no significant differences between the levels of HSP70 in all groups. At last, all of the data got from all criteria revealed that there were no significant differences between women and men. Conclusion: All the data revealed that increasing temperature triggers apoptotic cell death in blood leukocytes but this increasing did not affect the number of blood cells clearly. On the other hand, in this model, HSP70 expression is not triggered by changing of temperature.

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Apoptosis , hyperthermia , heat shock proteins , immunohistochemistry , programmed cell death

Kocatepe Medical Journal

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Kocatepe Medical Journal