Author/Authors
kiliç, neşe eskisehir osmangazi university - faculty of medicine - department of medical biochemistry, Turkey , akyüz, fahrettin eskisehir osmangazi university - faculty of medicine - department of medical biochemistry, Turkey , tekin, neslihan aksaray university - faculty of science and letters - department of biotechnology and molecular biology, Aksaray, Turkey
Title Of Article
POSSIBLE REGULATORY EFFECTS OF APPLICATION OF ALPHA LIPOIC ACID AND INSULIN AGAINST CADMIUM EXPOSED DIABETIC RATS
شماره ركورد
42325
Abstract
OBJECTIVE: Environmental exposure to the cadmium (Cd), is associated with hyperglycemia and reduced serum insulin. This investigation was planned to assess the effects of Lipoic Acid (LA) and insulin on glycolytic enzymes, liver marker enzymes and lipids in Cd exposed diabetic rats. MATERIAL AND METHODS: Male Wistar rats were separated into 7 groups (n=8 in each group). Groups were designed as control, diabetic control, diabetic + CdCl_2, diabetic + insulin, diabetic + CdCl_2 + insulin, diabetic + CdCl_2 + LA, anddiabetic + CdCl_2 + insulin + LA groups. Type 1 diabetes was established by intraperitoneal (i.p.) injection of streptozotocin (STZ) (65 mg/kg) into 6 groups. Insulin (4 IU/kg/day) was given subcutaneously (s.c.) to insulin treated groups. CdCl_2 (1,2 mg/kg/day) was given s.c. to CdCl_2 treated groups. LA (100 mg/kg/day) was given i.p. to LA treated groups. CdCl_2, LA, and insulin treatment were started 2 days after intraperitoneal STZ injection and continued for 3 weeks. Serum glucose, AST, ALT, BUN, LDL, HDL, and TG levels and liver hexokinase (HK), pyruvate kinase (PK), whole blood HbA1c level, and Na+/K+ATPase activity were evaluated. RESULTS: In diabetic group, serum glucose, HbA1c, TG, LDL, AST, ALT, ALP, and BUN levels were higher than control, but HDL was lower. In liver tissue, activities of Na+/ K+ATPase, HK and PK activities were decreased in diabetic control group. PK, HK and Na+/K+ATPase activities were increased in liver in diabetic+CdCl_2 and Diabetic+ Insulin+CdCl_2 groups. An increase was determined in activities of HK, PK, and Na+/K+ATPase in insulin and LA treated groups compared with diabetic control group. CONCLUSIONS: These results suggest that application of insulin and LA could be an effective therapeutic intervention against liver injury caused by Cd and STZ.
From Page
98
NaturalLanguageKeyword
Lipoic acid , Cadmium , Hexokinase , Pyruvate kinase , Na⁺ , K⁺ATPase
JournalTitle
Kocatepe Medical Journal
To Page
108
JournalTitle
Kocatepe Medical Journal
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