Author/Authors
ermurat, yakup abant izzet baysal üniversitesi - bilimsel endüstriyel ve teknolojik uygulama ve araştırma merkezi, Bolu, Turkey
Title Of Article
A REVIEW ON INTEIN MEDIATED RECOMBINANT PROTEIN PURIFICATION PROCESSES WITHOUT USE OF PROTEASE
شماره ركورد
44646
Abstract
In this review study, conducted researches on inteins and recombinant proteins to increase splitting activity of inteins that are utilised in recombinant protein purification processes without use of protease have been investigated. Researches on protein cleaving by use of inteins are aimed to make inteins and protein purification processes become more effective. The processes are accomplished in vivo and in vitro and intein and target protein included polyclonal Polymerase Chain Reaction (PCR) products are cloned than the expressed plasmids are fused to cloned strains. Kinetics of activity of cleaving and activity of cleaving rates were determined in different pH, temperature and filtration times. In the completed researches, protein cleaving activities of different microbial natural and engineered inteins have been investigated and among them the cleaving activity of recombinant Nostoc punctiforme (Npu) DnaE C-inteins were found to be very high. The studies are focused on the C-inteins due to their recepient abilities of the amino acid additions.
From Page
95
NaturalLanguageKeyword
Intein , Recombinant protein purification , Cloning , Kinetics
JournalTitle
Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology
To Page
102
JournalTitle
Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology
Link To Document